Objective: Gynecological cancers represent the fourth most common type of cancer worldwide. The high-performance liquidchromatography (HPLC) analysis revealed the presence of phenolic compounds, including catechin, epicatechin, taxifolin andphenolic acids in pine bark. The anticarcinogenic, antiproliferative and apoptotic effects of pine bark extracts on the human ovariancancer cell line (MDAH-2774) were investigated.Materials and Methods: Gene expression was determined by means of the real-time reverse transcription-polymerase chain reaction(RT-PCR) method.
A TdT-mediated dUTP Nick-End labelling (TUNEL) test was conducted to ascertain the extent of apoptosis. TheIC₅₀ dose in MDAH-2774 cells was determined to be 117.6 μg/ml by 48 hours.Results: Notable alterations were discerned in the expression of caspase 3, 9, and 10 genes, which are implicated in programmed celldeath, as well as in genes such as Bax, Bcl-2, and Bcl-Xl, which play a pivotal role in regulating the mitochondrial apoptosis pathway.
Anotable increase in the proportion of apoptotic cells was observed in the dose group. A significant reduction in invasion capacity wasalso identified. It has been demonstrated that pine bark extract can inhibit the migration capacity of ovarian cancer cells and colonyformation.Conclusion: The findings suggest that pine bark extract exerts anticarcinogenic, antiproliferative and apoptotic effects on the ovariancancer cell line.