Objective: Lung cancer remains one of the leading causes of cancer-related mortality worldwide, and the emergence of resistance to existing therapies together with severe adverse effects necessitates the investigation of novel therapeutic agents. In this study, the effects of bee venom (BV) derived from Apis mellifera anatoliaca on the non-small cell lung cancer cell line A549 were evaluated with respect to cell viability and cell migration.Methods and Results: Cell viability was assessed using the MTT assay following treatment with BV at concentrations of 8, 16, and 32 ng/mL.
At the sixth hour, statistically significant differences were detected among the experimental groups (p 0.05); however, a limited but significant difference was observed at the 24th hour (p = 0.02). According to two-way repeated-measures ANOVA, BV concentration did not exert a significant main effect on cell viability (F(7,16) = 0.53; p = 0.80), whereas the time factor showed a strong and significant effect (F(3,41,54,52) = 146.3; p